PRODUCT

NAD+ (1000 Mg)

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Quickstart Highlights

NAD+ (nicotinamide adenine dinucleotide) is a critical coenzyme involved in cellular energy metabolism, DNA repair, and mitochondrial function. Clinical research has primarily used high-dose intravenous infusions, although lower-dose subcutaneous administration is emerging as a practical maintenance approach. This educational protocol presents a once-daily subcutaneous regimen with gradual titration to improve tolerability.

Reconstitution: Add 3.0 mL of bacteriostatic water to achieve a 333.3 mg/mL concentration.
Typical Daily Range: 50–100 mg once daily by subcutaneous injection (with gradual titration from lower doses).
Easy Measuring: At 333.3 mg/mL, 1 unit = 0.01 mL = 3.33 mg on a U-100 insulin syringe.
Storage: Lyophilized: store frozen at −20 °C (−4 °F). Reconstituted: refrigerate at 2–8 °C (35.6–46.4 °F) for up to 14 days. Protect from light and avoid freeze–thaw cycles.

Reconstitution

Allow the lyophilized vial to reach room temperature before opening to minimize moisture condensation.
Draw 3.0 mL of bacteriostatic water (0.9% benzyl alcohol) using a sterile syringe.
Inject the diluent slowly down the inside wall of the vial to avoid foaming; do not inject directly onto the powder.
Gently swirl or roll the vial until the powder is completely dissolved (do not shake vigorously).
The resulting solution should be clear and colorless. If discoloration or precipitate is present, discard the solution.
Label the vial with the reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light.
Use within 14 days and inspect the solution for clarity before each use.

Protocol Overview

Goal:Support cellular energy metabolism, DNA repair pathways, and mitochondrial function through exogenous NAD+ supplementation
Schedule:Daily subcutaneous injections for 8–16 weeks with gradual dose titration.
Dose Range:50–100 mg daily; start at 50 mg and increase by approximately 25 mg per week, as tolerated.
Reconstitution:3.0 mL per 1000 mg vial (333.3 mg/mL) for accurate unit measurements.
Storage:store frozen at −20 °C (−4 °F). Reconstituted: refrigerate at 2–8 °C (35.6–46.4 °F) for up to 14 days, protected from light.

Dosage Protocols

Start:50 mg por dia durante a Semana 1 para avaliar a tolerância individual.
Dose Escalation:Aumente para 75 mg por dia na Semana 2 e, se houver boa tolerância, avance para 100 mg por dia na Semana 3.
Maintenance:Continue com 100 mg por dia da Semana 3 até a Semana 16 (ou ajuste conforme a resposta e a tolerância).
Frequency:Uma vez ao dia, por injeção subcutânea.
Timing:Pode ser administrado em qualquer horário consistente do dia; algumas pessoas preferem a aplicação pela manhã.
Site Rotation:Faça a rotação sistemática dos locais de aplicação (abdômen, coxas e parte superior dos braços) para ajudar a evitar o acúmulo de tecido.

Storage Instructions

Lyophilized:Conservar a −20 °C (−4 °F) o menos (idealmente −80 °C para almacenamiento de varios años) en un lugar seco y protegido de la luz. Minimice la exposición a la humedad, ya que el polvo de NAD+ es higroscópico.
Reconstituted:Conservar refrigerada entre 2–8 °C (35.6–46.4 °F) y utilizar dentro de aproximadamente 14 días. Mantener protegida de la luz, ya que la radiación UV puede degradar el NAD+.

Important Notes

Practical considerations for consistency, safety, and optimal results.

Use a new sterile insulin syringe for each injection and dispose of used needles in an appropriate sharps container.
Rotate injection sites systematically (abdomen at least 2 inches from the navel, outer thighs, and the back of the upper arms) to reduce soreness and prevent lipohypertrophy.
Clean both the vial stopper and the injection site with alcohol swabs, allowing the skin to air-dry before injection.
Inject slowly over 5–10 seconds to minimize tissue irritation, then wait a few seconds before withdrawing the needle.
Record the daily dose, injection site, and any observed effects to maintain consistency and monitor tolerance.
If persistent redness or a small lump develops at an injection site, apply an over-the-counter hydrocortisone cream and rotate to a different location.
Starting at the lower dose (50 mg) is recommended to assess individual tolerance before increasing the dose.

How It Works

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme essential for redox reactions, cellular energy metabolism (glycolysis, the TCA cycle, and oxidative phosphorylation), and cellular maintenance processes such as DNA repair and mitochondrial biogenesis. Cellular NAD+ levels naturally decline with age and metabolic stress, which may contribute to reduced mitochondrial function and diminished cellular resilience. Clinical research on NAD+ therapy has primarily focused on high-dose intravenous (IV) infusions (500–1000 mg) for applications such as addiction treatment and acute metabolic support. A pilot study demonstrated that a 750 mg IV infusion administered over 6 hours was well tolerated in humans, with rapid metabolic clearance and no evidence of acute toxicity. However, intravenous administration requires medical supervision and specialized equipment. Lower-dose subcutaneous (SC) and intramuscular (IM) injections are emerging as practical alternatives for maintenance therapy and wellness applications. Compounded NAD+ can be administered subcutaneously in small volumes, making self-administration more convenient for ongoing use. Conservative protocols typically begin with 50–100 mg per injection several times per week, while this protocol recommends daily subcutaneous administration with gradual dose titration to optimize individual tolerance and response.

Lifestyle Factors

Nutrition: Consume a balanced diet rich in NAD+ precursors (niacin/vitamin B3 and tryptophan) along with supportive nutrients such as B vitamins and magnesium. Consider foods that support methylation pathways.
Exercise: Regular physical activity, including both resistance training and aerobic exercise, naturally enhances cellular NAD+ biosynthesis and supports mitochondrial adaptations.
Sleep: Prioritize 7–9 hours of quality sleep each night to promote cellular repair and optimize circadian NAD+ metabolism.
Stress Management: Chronic stress can deplete NAD+ stores through activation of poly(ADP-ribose) polymerases (PARPs). Incorporate stress-reduction practices such as meditation or yoga.
Avoid Excessive Alcohol: Alcohol metabolism consumes NAD+; limit or avoid alcohol intake while following NAD+ protocols.

Injection Technique

Wash your hands and clean both the vial stopper and injection site with alcohol.
Allow the skin to air-dry completely.
Use a U-100 insulin syringe (28–31G).
Pinch the skin and insert the needle at a 45° angle (90° with ultrashort needles).
Inject slowly over 5–10 seconds. Do not aspirate.
Wait a few seconds before removing the needle and dispose of it in a sharps container.
Rotate between the abdomen, thighs, and back of the upper arms to reduce irritation and prevent lipohypertrophy.

NAD+ (1000 Mg)

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