PRODUCT

5-Amino-1MQ (10 mg Vial)

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Quickstart Highlights

5-Amino-1MQ dosage protocols center on this selective, cell-permeable NNMT (Nicotinamide N-methyltransferase) inhibitor studied for its potential to support fat metabolism, preserve lean muscle mass, and elevate intracellular NAD+ levels[1][2]. By blocking NNMT, 5-Amino-1MQ may help restore cellular energy balance and activate SIRT1 pathways associated with metabolic efficiency[3]. This educational protocol presents a subcutaneous injection approach to maximize bioavailability from the 10 mg vial format.

Reconstitute: Add 2.0 mL bacteriostatic water → 5 mg/mL concentration.
Typical daily range: 2.5–5 mg once or twice daily (subcutaneous).
Easy measuring: At 5 mg/mL, 1 unit = 0.01 mL = 50 mcg (0.05 mg) on a U-100 insulin syringe.
Storage: Lyophilized: freeze at −20 °C (−4 °F); after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) and use within 2–4 weeks[4].

Research Indications

Longevity

effective

NAD+ Enhancement
Significantly increases cellular NAD+ levels by inhibiting NNMT, supporting cellular energy production and DNA repair mechanisms
Mitochondrial Function
Improves mitochondrial biogenesis and efficiency, enhancing cellular energy production and reducing oxidative stress
Cellular Repair
Enhanced NAD+ availability supports sirtuins and other longevity pathways involved in DNA repair and cellular maintenance

Metabolism

effective

Energy Metabolism
Improves cellular energy production through enhanced mitochondrial function and more efficient glucose and fatty acid utilization
Insulin Sensitivity
May improve insulin sensitivity and glucose metabolism through enhanced cellular energy processes
Fat Oxidation
Potentially enhances fat burning and metabolic flexibility through improved mitochondrial function

Weight Loss

moderate

Metabolic Enhancement
Increases basal metabolic rate and helps body burn fat for energy while maintaining muscle mass preferentially
Fat Cell Reduction
Mouse studies (PMC5826726) show 30% adipocyte size reduction at 60mg/kg/day
Energy Balance
Shifts body's energy balance toward burning fat rather than storing it, particularly beneficial for obesity

Reconstitution

Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes .
Draw 2.0 mL bacteriostatic water with a sterile syringe.
Inject slowly down the vial wall; avoid foaming.
Gently swirl/roll until dissolved—solution should be clear (do not shake).
Label and refrigerate at 2–8 °C (35.6–46.4 °F) , protected from light; use within 2–4 weeks.
Remove the vial from freezer storage (−20 °C / −4 °F) and allow to equilibrate at room temperature for 15–20 minutes.
Label and refrigerate at 2–8 °C (35.6–46.4 °F), protected from light; use within 2–4 weeks.

Interactions

NAD+ Precursors (NMN, NR)

Synergistic

May work synergistically with NAD+ precursors by preventing breakdown while supporting production

Resveratrol

Synergistic

Both support longevity pathways; resveratrol activates sirtuins while 5-Amino-1MQ provides NAD+ substrate

Metformin

Monitor Combination

Both affect metabolic pathways; combination may enhance effects but requires careful monitoring

BPC-157

Compatible

No known interactions; different mechanisms of action with potential complementary benefits

Blood Thinners

Unknown

Limited interaction data; consult healthcare provider before combining with anticoagulants

Berberine

Monitor Combination

Both affect metabolic pathways; potential additive effects on glucose metabolism

Protocol Overview

Goal:Support metabolic efficiency through NNMT inhibition, potentially enhancing fat oxidation and NAD+ levels[1][2].
Schedule:Daily subcutaneous injections; each 10 mg vial provides 2–4 days of material.
Dose Range:2.5–5 mg once or twice daily.
Reconstitution:2.0 mL per 10 mg vial (5 mg/mL) for accurate measurements.
Storage:Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F).

Dosage Protocols

Start:2.5 mg once daily to assess tolerance.
Target:5 mg once daily or 2.5 mg twice daily (BID).
Frequency:Once or twice per day (subcutaneous).
Timing:Morning administration preferred; BID dosing may be split AM/PM.

Dosage Protocols

Conservative starting:150-250mcg1x daily
Conservative standard:250-500mcg1x daily
Conservative moderate:500mcg1x daily
Mouse study (PMC5826726):20mg/kg TID (60mg/kg/day)3x daily
Human equivalent (FDA scaling):~5mg/kg/day (~350mg for 70kg)Theoretical

Route:injectable

Storage Instructions

Lyophilized:Store at −20 °C (−4 °F) in dry, dark conditions; stable up to 24 months[4].
Reconstituted:Refrigerate at 2–8 °C (35.6–46.4 °F); use within 2–4 weeks.

Important Notes

Practical considerations for consistency and safety.

Use new sterile insulin syringes for each administration; dispose in a sharps container[6].
Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation[7].
Inject slowly; a mild stinging sensation may occur due to the quinolinium structure.
Document daily dose and site rotation to maintain consistency.
The 10 mg vial format requires multiple vials for extended protocols; plan supplies accordingly.

Reconstitution

Confirm which protocol (mcg vs mg) you're following
Clean vial stopper, inject BAC water slowly down side
Gently swirl until dissolved (never shake)
Label clearly with concentration AND units (mg/mL)
Store in refrigerator, use within 28 days

Route:injectable

How It Works

5-Amino-1MQ (5-amino-1-methylquinolinium) is a synthetic small molecule that selectively inhibits Nicotinamide N-methyltransferase (NNMT)[1]. NNMT is a cytosolic enzyme that methylates nicotinamide (vitamin B3) using S-adenosylmethionine (SAM) as a methyl donor. In states of obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and reducing NAD+ availability[2]. By inhibiting NNMT, 5-Amino-1MQ may spare nicotinamide for NAD+ synthesis via the salvage pathway, thereby activating SIRT1 (Sirtuin 1) pathways associated with mitochondrial biogenesis, fat oxidation, and metabolic flexibility[3]. Preclinical studies indicate that NNMT knockdown or inhibition can protect against diet-induced weight gain and improve metabolic parameters without reducing food intake[8][9].

Lifestyle Factors

Pair with a balanced, protein-forward diet tailored to energy needs.
Combine resistance training and aerobic activity—preclinical data suggest synergy with exercise[10].
Prioritize sleep and stress management to support metabolic adaptation.
Consider complementary NAD+ precursors (NMN/NR) per emerging stack protocols[11].

Injection Technique

Clean the vial stopper and skin with alcohol; allow to dry.
Pinch a skinfold; insert the needle at 45–90° into subcutaneous tissue[12][13].
Do not aspirate for subcutaneous injections; inject slowly and steadily[12].
Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy[7].
Slow injection may minimize any stinging sensation associated with the compound.

Benefits and Side Effects

Benefits

May support reductions in fat mass while preserving lean muscle in animal models[8][9].
Associated with elevated NAD+ levels and SIRT1 activation in preclinical studies[2][3].
Enhanced grip strength observed in aged mice when combined with exercise[10].

Side Effects

Generally well tolerated; occasional reports of mild headache, transient jitteriness, or injection-site reactions.
Long-term human safety data not established; this compound remains investigational.

5-Amino-1MQ (10 mg Vial)

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